Human high-sensitivity C-reactive protein (hs-CRP) elisa Kit Instruction Manual Procedure 1. Dilution and Addition of Standards: Place 10 wells in standard plate wells on the ELISA plate, in the first and second wells. Add 100 μl of the standard respectively, and then add 50 μl of the standard diluent to the first and second wells and mix. Then add 100 μl from the first well and the second well to the third well and the fourth well respectively. Add 50 μl of the standard diluent to each of the third and fourth wells and mix. Then remove 50 μl from each of the third and fourth wells. Discard each and add 50 μl to the fifth and sixth wells. In the fifth and sixth wells, 50 μl of the standard diluent was added and mixed; 50 μl of each of the fifth and sixth wells was added to the seventh and eighth wells after mixing, and the seventh and eighth wells were added. Add 50 μl of standard diluent to the wells. Mix 50 μl of the seventh and eighth wells and add them to the ninth and tenth wells. Add the standard diluent to the ninth and tenth wells respectively. After homogenization, remove 50 μl from each of the ninth and tenth wells. (After dilution, the volume of each well was 50 μl, and the concentrations were 1800 ng/L, 1200 ng/L, 600 ng/L, 300 ng/L, and 150 ng/L, respectively). 2. Samples: blank wells (blank control wells without sample and enzyme labeling reagents, and the rest of the steps are the same), sample wells to be tested. 40 μl of sample diluent is added to the well of the sample to be tested on the enzyme-labeled plate, and then 10 μl of the sample to be tested is added (the final dilution of the sample is 5 times). Add the sample to the bottom of the wells of the microtiter plate, try not to touch the wall of the well, mix gently by shaking. 3. Incubation: Seal the plate with a cover plate and incubate at 37°C for 30 minutes. 4. Dosing solution: 30 times (20 times the 48T) diluted washing solution is diluted with distilled water 30 (20 times of 48T) before use. 5. Washing: Carefully remove the sealing film, discard the liquid, spin dry, fill the well with washing liquid, and let it stand for 30 seconds. Discard it. Repeat 5 times and pat dry. 6. Enzyme addition: Add 50 μl of Enzyme Labeling Reagent to each well, except blank wells. 7. Incubation: Operate in the same way as in 3. 8. Washing: Operate with 5. 9. Coloration: Add 50 μl of color reagent A to each well, add 50 μl of color reagent B, mix gently, and avoid color at 37°C 15 minutes. 10. Termination: Add 50 μl of stop solution to each well and stop the reaction (in this case the blue turns to yellow). 11. Measurement: The absorbance (OD value) of each well was measured sequentially with a blank air conditioner at zero wavelength of 450 nm. The measurement should be performed within 15 minutes after adding the stop solution. [human high-sensitivity C-reactive protein (hs-CRP) elisa kit] Notes: 1. The kit should be removed from a refrigerated environment and allowed to equilibrate for 15-30 minutes at room temperature. If the ELISA plate is unopened after opening, the slats should be stored in a sealed bag. 2. The concentrated washing solution may have crystals precipitated, and may be heated and dissolved in a water bath during dilution. The washing does not affect the results. 3. The sampler should be used for each step, and the accuracy of the sampler should always be calibrated to avoid experimental errors. It is best to control the injection time within 5 minutes at a time. If the number of specimens is large, it is recommended to use a sample of guns. 4. Please make a standard curve at the same time for each measurement. It is best to make a duplicate hole. If the content of the test substance in the sample is too high (the OD value of the sample is larger than the OD value of the first hole of the standard product hole), please dilute a certain multiple (n times) with the sample diluent and then measure. Calculate the total dilution by multiplying Multiple (×n×5). 5. Sealing film is limited to one-time use to avoid cross-contamination. 6. Protect the substrate from light. 7. In strict accordance with the instructions of the operation, the test results must be determined by the microplate reader. 8. All samples, washing liquids and all kinds of waste should be handled as infectious agents. 9. The lot number components of this reagent must not be mixed. 10. If there is a difference with the English manual, the English manual shall prevail. Performance of the kit: 1. The correlation coefficient between the linear regression of the sample and the expected concentration is 0.95 or more. 2. Less than 9% and 11% in the batch and inspection respectively. Detection range: 0.2IU/L - 6IU/L The company specializes in distributing domestic and foreign well-known brand ELISA kits. All kinds of specimens, species, and specific indicators are welcome. Consultation,