Manual of human complement 5a (C5a) elisa kit
Elisa kit specifications: 48-well configuration / 96-well configuration
Standard diluent: 1.5ml × 1 bottle
Enzyme label reagent: 3 ml × 1 bottle (48) / 6 ml × 1 bottle (96)
[Human complement fragment 5a (C5a) elisa kit] This reagent is for research use only
Calculation:
Taking the concentration of the standard as the abscissa and the OD value as the ordinate, draw a standard curve on the coordinate paper, and find the corresponding concentration from the standard curve according to the OD value of the sample; then multiply by the dilution factor; Calculate the linear regression equation of the standard curve with the OD value, substitute the OD value of the sample into the equation, calculate the sample concentration, and multiply it by the dilution factor to obtain the actual concentration of the sample.
Kit composition:
Sealing film: 2 pieces (48) / 2 pieces (96)
Instructions: 1 copy
Sealed bag: 1
Standard product: 2700ng / L 0.5ml × 1 bottle 0.5ml × 1 bottle Store at 2-8 ℃
Enzyme-coated plate: 1 × 48 1 × 96 2-8 ℃
Sample diluent: 3ml × 1 bottle 6ml × 1 bottle Store at 2-8 ℃
Developer A solution: 3ml × 1 bottle 6 ml × 1 bottle Store at 2-8 ℃
Developer B: 3ml × 1 bottle 6 ml × 1 bottle Store at 2-8 ℃
Stop solution: 3ml × 1 bottle 6ml × 1 bottle Store at 2-8 ℃
Concentrated washing solution: (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle Store at 2-8 ℃
Experimental principle:
This kit uses the double antibody sandwich method to determine the level of human complement 5a (C5a) in the specimen. Microporous plates were coated with purified human complement fragment 5a (C5a) antibody to prepare solid-phase antibodies. Complement fragment 5a (C5a) was added to the monoclonal antibody-coated microwells in turn, followed by HRP labeled complement fragment 5a (C5a ) The antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the complement segment 5a (C5a) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human complement fragment 5a (C5a) in the sample was calculated by a standard curve.
Purpose: This kit is used to determine the content of complement 5a (C5a) in human serum, plasma and related liquid samples.
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