Human mesenchymal stem cells are cells required for bone tissue engineering due to their inherent pluripotency. Unfortunately, they have a high degree of variability because they rapidly undergo replicative senescence in primary cultures in vitro ( replicative senescence), so when they continue to proliferate in vitro, they lose their proliferation potential. To this end, the researchers sought a way to reduce the variability of these cells.
In the current new study, from the David Geffen School of Medicine at University of California, Los Angeles and the Veterans Administration Greater Los Angeles Healthcare System) DS Bischoff, NS Makhigani and DT Yamaguchi used lentivirus to insert a gene called human telomerase reverse transcriptase (TERT) into human mesenchymal stem cells. They provided evidence It was confirmed that human TERT can enhance the growth ability of human mesenchymal stem cells. In addition, they confirmed that they can construct a stable human mesenchymal stem cell line, that is, they can be cultured in vitro and can perform repeated cell divisions, and at the same time confirm this stem cell Ability to differentiate into osteoblasts for potential use in bone tissue engineering.
Dr. Jane Taylor, editor-in-chief of BioResearch Open Access and the British Medical Research Council (MRC Centre for Regenerative Medicine) at the University of Edinburgh, Scotland, said, "We have constructed a stable human mesenchymal stem cell while maintaining differentiation into osteoblasts and The potential of adipocytes brings important benefits to bone tissue engineering research, especially for studies that require large numbers of cells. "
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