Shanghai Xinzhuang Instrument Co., Ltd. offers solid phase extraction (SPE) as a physical separation technique that involves both liquid and solid phases. In this process, the solid adsorbent has a stronger affinity for the target compound than the solvent used to dissolve the sample. As the sample solution flows through the adsorbent bed, the desired isolate is retained on the surface of the adsorbent while other components pass through. This allows for the isolation of high-purity and concentrated compounds by selectively adsorbing only the target substance.
1. **Capacity and Selectivity**: The capacity of an adsorbent refers to the maximum amount of analyte it can retain under optimal conditions. Different bonded silica-based adsorbents have varying capacities depending on their structure and properties. Selectivity, on the other hand, is the ability of the adsorbent to distinguish between the target compound and other matrix components. A highly selective adsorbent retains only the desired analyte, effectively removing impurities. This selectivity depends on three key factors: the chemical nature of the analyte, the characteristics of the adsorbent, and the composition of the sample matrix.
2. **Retention and Elution**: A common approach in SPE is to use a syringe-like column filled with solid adsorbent. As the sample solution passes through, the analyte may either be retained or eluted based on its interaction with the adsorbent. Retention occurs when the analyte is attracted to the adsorbent surface, preventing it from moving forward. This behavior is influenced by the analyte, the solvent, and the adsorbent itself. Elution is the process of washing the retained analyte off the adsorbent using a solvent that has a stronger affinity for the analyte than the adsorbent.
3. **Basic Procedure of Solid Phase Extraction**: The process begins with the introduction of the sample containing both the target compound and interfering substances. The adsorbent selectively retains the analyte and some interferences, while others flow through. A suitable rinsing solvent is then used to remove the retained interferences without disturbing the analyte. Finally, the purified and concentrated analyte is eluted from the adsorbent for further analysis.
Shanghai Xinzhuang Instrument Co., Ltd. specializes in manufacturing a wide range of laboratory instruments. With a team of highly skilled professionals and experienced technical support staff, the company provides comprehensive solutions from design and development to installation and commissioning. Our senior engineers are dedicated to delivering high-quality products and services tailored to meet your needs. We welcome both new and returning customers to reach out and learn more about our offerings.
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