Today, Shanghai Hengyuan Xiaobian has compiled a list of reasons why the sensitivity of the ELISA kit is low. I hope to help you:
1. The time in transit is too long, the temperature is too high, and the transportation time should be shortened as much as possible. In summer, ice should be placed to cool down.
2. The kit is not well balanced. After removing the kit from the refrigerator at 2-8 °C, open the lid and equilibrate for at least 20 minutes at room temperature to ensure that all reagents are equilibrated to room temperature (about 25 ° C).
3. The temperature of the incubator is less than 37 °C. Pay attention to the temperature of the incubator. After placing the reaction plate, try to reduce the number of opening times to avoid affecting the temperature. The non-isolated incubator should pay special attention.
4. If the holding time is insufficient, the accurate timing of the fixed clock should be corrected.
5. The impact force during washing is too large, the soaking time is too long, the washing time is increased, the washing time is kept according to the instructions, and the washing times are accurately remembered.
6. The pipette has insufficient liquid absorption, too much water is hanged on the inner wall of the nozzle or the inner wall is not clean. The pipette should be calibrated. The nozzle should be matched. The nozzle should be tight when it is installed. The inner wall of the nozzle should be clean. use.
7. There is a problem with the quality of distilled water. Use freshly qualified distilled water.
8, the substrate action time is insufficient, quasi-deterministic.
Then share the method of storing and thawing serum without compromising product quality:
1. After taking the serum out of the freezer, it is first placed in a refrigerator at 2-8 °C to melt it, and then it is fully melted at room temperature, but it must be noted that it must be shaken regularly and uniformly during the melting process.
2. When stored at 2-8 °C for a long time, various proteins and lipoproteins (such as cold agglutinin, fibrinogen, villogen, etc.) in the serum may aggregate to form a precipitate or visible turbidity. Therefore, it is recommended that Store serum below -20 °C and avoid repeated freezing and thawing.
3. It is recommended that the serum should be stored at -2O °C. If stored at 4 °C, do not exceed one month. If one bottle cannot be used at one time, it is recommended to aseptically pack the serum into the appropriate sterilization container and put it back into the frozen. .
Shanghai Hengyuan Bio-Quality Supply ELISA Kit focuses on scientific research and is favored and supported by many researchers with its moderate price, fast delivery and excellent quality. In order to better reward customers, we will continue to provide better quality for scientific research units. More reliable research products, help your scientific research, the company insists on looking into the future, continue to create products in the field of biology, you are welcome to call to discuss business!
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