**Human Monoamine Oxidase (MAO) ELISA Kit – Instructions for Use**
This ELISA kit is designed for the quantitative determination of Human Monoamine Oxidase (MAO) in various biological samples. It is intended for research use only and should not be used for diagnostic or therapeutic purposes.
**Kit Specifications:**
- **Configuration:** 48-well or 96-well format
- **Standard Dilution:** 1.5 mL × 1 vial
- **Enzyme Standard Reagent:** 3 mL × 1 vial (for 48-well) / 6 mL × 1 vial (for 96-well)
- **Sealing Film:** 2 pieces (for 48-well) / 2 pieces (for 96-well)
**Procedure Overview:**
1. Prepare the standard solutions according to the instructions provided in the kit manual.
2. Add the prepared standards, controls, and samples to the appropriate wells of the microplate.
3. Incubate the plate as specified in the protocol.
4. Wash the plate to remove unbound components.
5. Add the enzyme conjugate and incubate again.
6. Wash the plate once more.
7. Add the substrate solution and incubate until a color develops.
8. Stop the reaction with the stop solution.
9. Measure the optical density (OD) at 450 nm using a microplate reader.
**Data Analysis:**
- Plot the standard curve by using the concentration of the standards on the x-axis and the corresponding OD values on the y-axis.
- Draw the standard curve on graph paper or use software to generate a linear regression equation.
- Determine the OD value of each sample and input it into the equation to calculate the corresponding concentration.
- Multiply the calculated concentration by the dilution factor (if applicable) to obtain the actual sample concentration.
**Important Notes:**
- Always perform the assay in duplicate or triplicate to ensure accuracy.
- Ensure all reagents are brought to room temperature before use.
- Avoid contamination during the procedure.
- Store unused reagents at the recommended temperature as indicated in the kit manual.
**Storage Conditions:**
- Store the kit at 2–8°C when not in use.
- Do not freeze the reagents unless specified in the manual.
This kit provides a reliable and sensitive method for measuring MAO levels, making it an essential tool in biochemical and pharmacological research.
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