Phthalates are environmental endocrine disruptors and are a class of toxic organic pollutants that interfere with human and other animal endocrine. Phthalates in cosmetics are widely used in perfumes, nail polishes, detergents, etc. They are also used as solvents and aromatic fixatives for some products. Too much use of phthalate-containing cosmetics will increase the probability of women suffering from breast cancer, and can easily cause miscarriage and fetal malformations in pregnant women. The detection of phthalates in cosmetics is of great significance to protect consumers' health.
1. Test equipment
1. High performance liquid chromatograph
2. Vertical Oscillator: Tianjin Hengao Technology Development Co., Ltd.
3. Ultrasonic cleaner: Tianjin Hengao Technology Development Co., Ltd.
4. Filter: Tianjin Hengao Technology Development Co., Ltd.
Second, the test method
1. Chromatographic conditions
Column: C18 4.6mm × 250mm, 5μm
Detector: Diode array detector
Detection wavelength: 280nm
Flow rate: 1.0ml / min
Column temperature: 25 ℃
Injection volume: 5μl
2. Preparation of standard solution
Accurately weigh 0.05g of 10 kinds of phthalate standard products, dissolve with methanol, transfer to 50ml volumetric flask, dilute to the mark, shake well, and prepare a standard solution with a certain concentration. Pipette the standard solution 0.00, 1.00, 2.00, 4.00, 6.00, 8.00, 10.00ml accurately in a 10ml graduated test tube, dilute to the mark with the mobile phase, shake with a vertical shaker (can operate 6 or 10 samples at the same time) , To prepare a mixed standard solution with a certain mass concentration.
3. Sample preparation and determination
Accurately weigh 1.0g of the cosmetic sample, place it in a 10ml graduated graduated tube, add methanol to the mark, shake, and ultrasonically extract for 20 ~ 30min. Filter through a 0.45μm filter membrane. The filtrate is to be tested. Under the set chromatographic conditions, take 5 μl for HPLC analysis.
Three, calculation
According to the peak area, the mass concentration of 10 phthalate components was obtained from the standard curve. The final concentration of phthalate in the sample should be a multiple of dilution in concentration.
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