Purpose: This kit is used to determine the content of ferritin (FE) in rat serum, plasma and related liquid samples. Experimental principle This kit uses the double antibody sandwich method to determine the level of rat ferritin (FE) in the specimen. Microporous plates were coated with purified rat ferritin (FE) antibody to prepare solid-phase antibodies. Ferritin (FE) was added to the monoclonal antibody-coated microwells in turn, followed by HRP-labeled ferritin (FE) antibody Combine to form antibody-antigen-enzyme-labeled antibody complex. After thorough washing, add substrate TMB to develop color. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the ferritin (FE) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of rat ferritin (FE) in the sample was calculated by a standard curve. Kit composition 1 30 times concentrated washing solution 20ml × 1 bottle 7 stop solution 6ml × 1 bottle 2 enzyme label reagent 6ml × 1 bottle 8 standard (800ng / ml) 0.5ml × 1 bottle 3 enzyme label coated plate 12 well 8 strips 9 standard diluent 1.5ml × 1 bottle 4 sample diluent 6ml × 1 bottle 10 manual 1 copy 5 developer A solution 6ml × 1 bottle 11 sealing film 2 sheets 6 developer B solution 6ml × 1 / Bottle 12 sealed bag 1
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