Isolation and purification of soluble antigen

Faced with new problems and new challenges in food safety, many new views and new models on food safety management and control have emerged in various countries and regions. In order to meet the requirements of these new views and new models, many adaptations have emerged. Food safety detection technology and new methods. There are many food detection technologies. Among them, biological detection methods are widely used in their fields, and immunological methods are widely used in biological detection methods. The basic principle is to use the specificity of antibodies and antigens. Combined with characteristics to achieve the method of detecting contaminants in food.

According to the basic principles of immunology, the preparation process of antigens occupies an important position in this method. Antigens can be divided into water-insoluble particulate antigens and soluble colloidal antigens according to water solubility. Among them, there are many types of soluble colloidal antigens, mainly including proteins, polysaccharides and Bacterial toxins, etc., these substances can exist extracellularly and intracellularly, and are mixed with other substances, so appropriate methods must be adopted for separation and purification. For extracellular antigen cells only need to be collected and then separated and purified, and Intracellular antigens need to be broken up before separation and purification.