It's only one week left in this month, and we're wrapping up our latest technical insights on the classic ELISA kit. Our expert has conducted an experimental analysis and summary of the blocking step, which is a crucial part of the ELISA procedure. Understanding the right blocking agents can significantly improve your results and reduce background noise.
Commonly used blocking agents include 0.05%-0.5% BSA, 10% calf serum, or 1% gelatin. Skim milk powder is also widely used because it's cost-effective and can be applied at higher concentrations, such as 5%-10%. Other options like casein or rare animal sera are also available. However, it's important to note that theoretical knowledge alone isn't enough—practical testing is essential. Always test different blocking agents to see which works best for your specific experiment. The final choice should always be based on your own test results and conditions.
For coating solutions, common choices include phosphate buffer at pH 7.2, Tris-HCl buffer at pH 7-8, and carbonate buffer at pH 9.6. The blocking step involves filling unoccupied sites on the plate with unrelated proteins, preventing non-specific binding of interfering substances during later steps. In some cases, especially when maintaining the original coating specificity is important, a neutral buffer solution may be used instead.
This concludes today’s technical tip. If you want to learn more about ELISA kits and related techniques, feel free to contact our company directly or leave a message on our website. We’re here to help you succeed in your experiments. Stay tuned for more useful tips next time!
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