Separation and Preparation of Salvianolic Acid B by High Speed ​​Countercurrent Chromatography

The high-speed counter-current chromatography was used to separate and purify salvianolic acid, a water-soluble component of salvia miltiorrhiza, to prepare salvianolic acid B chemical reference substance. The solvent system used for separation is n-hexane 2 ethyl acetate 2 water 2 methanol (1.5: 5: 5: 1.5), the upper phase is used as the stationary phase, the lower phase is used as the mobile phase, the flow rate is 1.7mL / min, the instrument speed is 850rpm, The sample volume was 80 mg, and the purity was determined by HPLC. The results show that: 63.4mg salvianolic acid B can be prepared in one separation, and its purity is 98.6%. The method is simple to operate and can be used as a preparation and separation method for high-purity salvianolic acid B chemical reference substance.

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