Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) ELISA Test Kit User Manual The Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) ELISA Kit can only be used for scientific research, not for medical diagnostic testing Principle: The kit uses double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells pre-coated with granulocyte macrophage colony stimulating factor (GM-CSF) antibody, add the specimen, standard, and HRP-labeled detection antibody in sequence, incubate and wash thoroughly. The color is developed with the substrate TMB, which is converted into blue under the catalysis of peroxidase and into the final yellow under the action of acid. The color depth is positively correlated with the granulocyte macrophage colony stimulating factor (GM-CSF) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the sample concentration was calculated. Sample collection, processing and storage methods: 1. Serum: Use test tubes that do not contain pyrogens and endotoxins. Avoid any cell stimulation during the operation. After collecting blood, centrifuge at 3000 rpm for 10 minutes to quickly separate the serum and red blood cells carefully. 2. Plasma: EDTA, citrate or heparin anticoagulation. Take the supernatant by centrifugation at 3000 rpm for 30 minutes. 3. Cell supernatant: centrifuge at 3000 rpm for 10 minutes to remove particles and polymers. 4. Tissue homogenate: The tissue is crushed by adding appropriate amount of normal saline. Take the supernatant by centrifugation at 3000 rpm for 10 minutes. 5. Preservation: If the sample is not tested in time after collection, please aliquot it in one dose and freeze it at -20 ℃ to avoid repeated freezing and thawing. Thaw at room temperature and ensure that the sample is thawed evenly and fully. Self-provided items: 1. Microplate reader (450nm) 2. High-precision sampler and pipette tip: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL Store the box at 2-8 ° C and equilibrate at room temperature for 20 minutes before use. The concentrated washing liquid taken out of the refrigerator will have crystals, which is a normal phenomenon. The water bath is heated to completely dissolve the crystals before use. 2. The slats not used in the experiment should be immediately returned to the ziplock bag, sealed (dried at low temperature) and stored. 3. S0 standard with a concentration of 0 can be regarded as a negative control or blank; the sample has been diluted 5 times when operated according to the instructions, and the final result is multiplied by 5 to be the actual concentration of the sample. 4. Carry out the incubation operation strictly in accordance with the time, amount of liquid and sequence indicated in the manual. 5. Shake all liquid components thoroughly before use. Kit composition: Name 96-well configuration 48-well configuration Remarks Microwell enzyme plate 12 wells × 8 strips 12 wells × 4 strips None; standard product 0.3mL * 6 tube 0.3mL * 6 tube none; sample dilution 6mL 3mL none Detection antibody-HRP 10mL 5mL None; 20 × Wash buffer 25mL 15mL Dilute according to the instructions; Substrate A 6mL 3mL None; Substrate B 6mL 3mL None; Stop solution 6mL 3mL None; Sealing film 2 sheets 2 sheets None; Instructions 1 part 1 part none; ziplock bag 1 part 1 none; Note: The concentration of the standard (S0-S5) is: 0, 30, 60, 120, 240, 480 pg / mL reagent preparation 20 × washing buffer Dilution: Distilled water is diluted 1:20, that is, 1 part of 20 × washing buffer plus 19 parts of distilled water. Plate washing method: 1. Automatic plate washing machine: Inject 350μL of washing solution into each well, soak for 1min, and wash the plate 5 times. 2. Manually wash the plate: throw away all the liquid in the hole, fill each hole with the washing liquid, leave the hole in the hole for 1min, shake off the liquid in the hole, pat dry on the absorbent paper, and wash the plate 5 times in this way. Operation steps: 1. Take out the required slats from the aluminum foil bag after equilibrating at room temperature for 20min. The remaining slats are sealed with a ziplock bag and put back at 4 ℃. 2. Set up standard wells and sample wells, and add 50μL of standard products of different concentrations to the standard wells; 3. Add 10μL of the sample to be tested to the sample wells, and then add 40μL of the sample diluent; no blank wells. 4. In addition to the blank wells, add 100 μL of horseradish peroxidase (HRP) -labeled detection antibody to each of the standard wells and sample wells, seal the reaction wells with a sealing plate, and incubate in a 37 ° C water bath or incubator 60min. 5. Discard the liquid, pat dry on absorbent paper, fill each well with washing liquid, let stand for 1min, shake off the washing liquid, pat dry on absorbent paper, and repeat washing the plate 5 times (you can also wash the plate with a washing machine). 6. Add 50 μL of substrate A and B to each well, and incubate at 37 ° C for 15 min in the dark. 7. Add 50μL of stop solution to each well, and measure the OD value of each well at 450nm within 15min. Judgment of results: Draw a standard curve: In the Excel worksheet, the standard product concentration is used as the abscissa, and the corresponding OD value is used as the ordinate. Kit performance: 1. Accuracy: The linear regression between the standard product and the expected concentration R value is greater than or equal to 0.9900. 2. Sensitivity: The minimum detection concentration is less than 1.0 pg / mL. 3. Specificity: does not cross-react with other soluble structural analogs. 4. Repeatability: The coefficients of variation within and between plates are less than 15%. 5. Storage: Store at 2-8 ℃, protected from light and moisture. 6. Validity: 6 months
PP Spunbond , Pet Spunbond , SS,SSS, SMS,SSMMS Non-woven Fabric, Hydrophilic Spunbond, Hydrophobic Spunbond , Spunbond For Medical Pad, Spunbond For Baby Diaper, Medical Protective Clothing Spunbond
Spunbond fabrics are produced by depositing extruded, spun filaments onto a collecting belt in a uniform random manner followed by bonding the fibers. The fibers are separated during the web laying process by air jets or electrostatic charges. The collecting surface is usually perforated to prevent the air stream from deflecting and carrying the fibers in an uncontrolled manner. Bonding imparts strength and integrity to the web by applying heated rolls or hot needles to partially melt the polymer and fuse the fibers together. Since molecular orientation increases the melting point, fibers that are not highly drawn can be used as thermal binding fibers. Spunbond products are employed in carpet backing, geotextiles, and disposable medical/hygiene products. Since the fabric production is combined with fiber production, the process is generally more economical than when using staple fiber to make nonwoven fabrics.
Isotactic polypropylene is the most widely used polymer for spunbond nonwovens production. It provides the highest yield (fiber per kilogram) and covering power at the lowest cost because of its low density. Considerable advances have been made in the manufacture of polypropylene resins and additives since the first spunbond polypropylene fabrics were commercialized in the 1960s. Although unstabilized polypropylene is rapidly degraded by UV light, improved stabilizers permit several years of outdoor exposure before fiber properties deteriorate. To reduce cost, scrap or polypropylene fibers of inferior quality may be repelletized and then blended in small amounts with fresh polymer to produce first grade spunbond fabrics. This is very advantageous and important in a highly competitive industry.
Spunbond polyester fabrics typically have more uniform properties than other structures of nonwoven. They are produced using medium to coarse dpf continous filament fibers. Spunbond polyester fabrics are supplied in widths ranging from 1" to 204" with basis weights ranging from 12 gsm to over 300 gsm.
Common characteristics include:
excellent thermal properties
superior moldabilty
very strong
high dimensional stability
inherently UV stable
good permeability
Spunbond Polypropylene fabrics are commonly made with fine to medium dpf continuous filament fibers. These fabrics are uniform and dimensionally stable. Polypropylene fabrics require additives for UV and heat stability. Spunbond polypropylene fabrics are supplied in widths ranging from 1" to 200" with basis weights ranging from 8 gsm to over 200 gsm.
Common characteristics include:
soft hand
economical
Spunbond Bico products can be produced using different ratios of two different filament fibers, PET/PP, PET/PE, PET/PA, and PET/CoPET. These products are supplied in widths ranging from 1" to 165" with basis weights ranging from 20 gsm to 250 gsm.
Common characteristics include:
weldable
moldable
high dimensional stability
good fiber distribution
good permeability
Spunbond
Spunbond Fabric,Pp Spunbond Nonwoven,Disposable Hygiene Products,Spunbond For Disposable Coverall
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