Comparison of Comasil and Inertex Packed Column Application
1. Experimental method
Cometro high performance liquid chromatography system;
Column: Comasil C18 250 * 4.6mm, 5μm;
Inertex C18 250 * 4.6mm, 5μm;
UV detector wavelength: 254nm;
Mobile phase: methanol-0.1% phosphoric acid solution (85:15);
Flow rate: 1.00ml / min;
Sample: Rhubarb for testing;
Injection volume: 10μL
2. Experimental results
1. Comasil C18 250 * 4.6mm, 5μm
Channel A Results
Retention Time
Area
Theoretical plates (USP)
Resolution (USP)
Asymmetry
5.977
149339
10689
0.00000
1.13328
7.522
121891
10483
5.88475
1.23162
12.165
164025
13386
12.99876
1.06898
15.613
845852
15638
7.49639
0.99464
21.723
128104
16536
10.39870
1.03773
2. Inertex C18 250 * 4.6mm, 5μm
Channel A Results
Retention Time
Area
Theoretical plates (USP)
Resolution (USP)
Asymmetry
4.117
148074
5737
0.00000
1.08738
4.640
117634
4895
2.16837
1.24753
6.450
170317
7035
6.31892
1.05674
7.927
820991
10009
4.72902
1.09947
10.508
126814
9842
6.97177
1.13304
3. Results and discussion
1. Comasil packing has ultra-high carbon loading and ultra-low metal ion content, therefore, Comasil C18 column has strong retention and separation ability; Inertex packing has a slightly lower carbon loading than Comasil packing, so Greatly reduces the retention time of compounds, which is very suitable for the analysis of strong retention compounds
2. Comasil and Inertex C18 columns can meet the requirements of the Pharmacopoeia. The five main components can be well separated and peak shape, but comparing the retention time of the two columns, the analysis time of Inertex packed columns is only Being half of the Comasil column can greatly improve the efficiency of the analysis work.
3. In summary, different chromatographic columns are suitable for different samples. The chromatographic column is not perfect but only suitable. For different samples, finding the most suitable chromatographic column can make the analysis work more effective.
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