GE Healthcare recently launched a new affinity chromatography medium Capto L, which is suitable for the capture of antibodies and antibody fragments. It combines a rigid, high-flow agarose matrix with a recombinant protein L ligand that binds to immunoglobulin. This ligand has a strong affinity for the variable region on the antibody Kappa light chain. Therefore, Capto L is suitable for capturing various antibody fragments, such as Fab, single chain antibodies (scFv) and domain antibodies (Dabs).
(Picture from GE Healthcare)
Monoclonal antibodies are usually purified using Protein A affinity chromatography. However, corresponding purification schemes for antibody fragments have been lacking. One of the reasons is the high diversity of antibody fragments, and the other reason is that the chromatography media currently available on the market do not meet the requirements of industrial-scale purification. The introduction of Capto L provides a basis for the purification platform method of this biomolecule category.
Compared with ion exchange and hydrophobic interaction chromatography, the affinity chromatography capture step has a very stable and versatile process. The optimization limit required for purification with Capto L is minimal, and higher purity can be obtained in one step. This obviously saves the cost, time and efficiency in the process. Compared with other methods, because the high selectivity of the affinity chromatography capture step will help to reduce the subsequent fine purification step, less selective purification media is used in production, which can also benefit from it.
With its highly dynamic binding capacity and high selectivity, Capto L improves the process economy and process design flexibility. Capto L is a BioProcess chromatography medium, which means that this medium is designed and developed for large-scale production of biopharmaceuticals and has received relevant supporting documents. These supports include validated production methods, reliable long-term media supply, safety and ease of operation, and management support documents (RSF) that assist process validation and documents submitted to regulatory authorities.
Capto L is available in large packages and prepacked columns, which can accelerate the screening and optimization of binding and elution conditions. Prepacked columns include PreDictor â„¢ 96-well culture plates and PreDictor Robo Column â„¢ devices, as well as HiTrap â„¢ and HiScreen â„¢ pre-packed chromatography columns. Request more information
Important performance characteristics of Capto L include:
• High dynamic binding capacity and wide range of antibody fragment selectivity
• Ensured a solution containing antibody fragment purification of K light chain
• Rigid base matrix that allows high flow rate and high productivity and low ligand leakage
• BioProcess ™ media with management support and assurance for commercial production supply
In order to ensure the comprehensiveness of the capture platform, when Capto L lacks affinity for antibody fragments, GE Healthcare has a large number of complementary affinity chromatography media to choose from: KappaSelect chromatography media is combined with KappaFab (constant region) And LambdaFabSelect chromatography medium is combined with LambdaFab (constant region). In addition, MabSelect â„¢ can be used to capture antibody fragments because of its affinity for heavy chain subtype VH3.
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