The biosafety cabinet workbench usually assembles pre-filters, high-efficiency filters, ventilators, power distribution devices, etc. in a box made of sheet metal to form an independent operation unit. After the fan is running, an inward protection airflow is formed, and the clean air filtered by the high-efficiency air at the top of the operation area flows vertically through the operation area to provide reliable protection for the product. The exhaust air is also passed through a high efficiency air filter to protect the environment from pollution. Therefore, the correct use of biological safety cabinets can effectively reduce the risk of infection by reducing laboratory infections caused by aerosol exposure and cross-contamination of cultures.
The biological safety cabinet's exhaust system is equipped with a high-efficiency air filter that effectively traps all known infectious agents. It can retain 99.97% for particles with a diameter of 0.3 μm and can withstand particles larger or smaller than 0.3 μm. 99.99% and ensure that the microbiological air is discharged from the safety cabinet. Therefore, the correct use of biosafety cabinets can effectively reduce laboratory pollution caused by aerosol exposure or spills, cross-contamination of cultures, reduce environmental and personal safety hazards, and effectively protect the health of laboratory personnel. With safety, reduce the occurrence of occupational exposure.
Strengthening the proper use of biosafety cabinets and avoiding the risk of infection is crucial. Proper installation and management of the biosafety cabinet is the key to ensuring the cleanliness of the work area of ​​the workbench. Strict rules and regulations are in place to strictly control the tools, materials and visitors used. In order to prevent external high-speed disturbance airflow (such as high-speed disturbance airflow from doors, windows, fans, tuyères, return air outlets, and compressed air) to the operating area. Therefore, the biosafety cabinet should be installed at a location that avoids frequent flow of people and cannot be installed at the entrance of the room ventilation system. If the laboratory has windows, it should always be kept closed to prevent airflow from directly blowing onto the operation port and exhaust filter on the front of the safety cabinet. Allow sufficient space around the perimeter (back and side) and power supply for safety cabinet installation to facilitate timely removal of waste, repair and replacement of filters, and reduce the risk of contamination of workers. Secondly, the operator must be professionally trained before they can be employed. The operator must wear clean overalls, caps and masks that are easy to sterilize with long fibers, and carry out Class I and II (A1, A2, B1, B2) biosafety. When working horizontally, you can wear a common lab coat. Biosafety cabinets are required to wear special individual protective clothing (laboratory clothing, gowns, jumpsuits, aprons) when performing Class III operations. Gloves should be placed on the outside of the gown. If necessary, wear sleeves with elastic bands to protect the operator's wrist. Some operations require wearing eyes (safety eyes, goggles), face masks and gas masks. In short, they should be at different levels. Protection requires the selection of appropriate personal protective equipment and types. During operation, the table top cannot store too many items. It is strictly forbidden to block the front and back of the style grille. If it is a necessity, the storage position should not be less than 100 mm from the return air outlet. During operation, the operator's arms slowly extend into the biosafety cabinet in a straight line and are at least 1 min still, so that the airflow in the cabinet is relatively stable before operation.
In summary, only understand the performance, structure, protection level and some precautions of the biosafety cabinet, and correctly use the biosafety cabinet to select the biosafety cabinet suitable for the laboratory for your laboratory to protect the experimental environment from pollution. Effectively reduce the incidence of laboratory infections caused by aerosol exposure or the spread of harmful gases, and avoid the risk of infection.
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