Shanghai Haozhuang Instrument is a leading manufacturer and supplier of solid phase extraction (SPE) devices, offering a wide range of high-quality solutions for sample preparation in various scientific fields. SPE has become an essential technique in modern analytical chemistry since its development in the mid-1980s, playing a critical role in pharmaceutical, biomedical, environmental, and food analysis applications.
At its core, solid phase extraction is a physical process that involves the interaction between a liquid sample and a solid adsorbent. The principle relies on the selective adsorption of target compounds onto the surface of the adsorbent while other components pass through. This allows for the concentration and purification of analytes before further analysis.
The process typically includes several key steps: sample loading, washing, and elution. During sample loading, the analyte is retained by the adsorbent based on its chemical properties and the nature of the stationary phase. Washing removes impurities, and elution uses a solvent to release the target compound from the adsorbent, resulting in a purified and concentrated sample.
There are different types of SPE, including normal-phase, reverse-phase, and ion-exchange. Normal-phase SPE uses polar adsorbents and is suitable for polar compounds, while reverse-phase SPE employs non-polar materials for separating non-polar or weakly polar substances. Ion-exchange SPE works based on electrostatic interactions and is ideal for charged molecules.
To ensure optimal performance, it's important to select the right SPE cartridge or filter membrane based on the sample matrix and analyte characteristics. Proper conditioning of the adsorbent with solvents like methanol helps improve retention and reproducibility. Sample preparation techniques such as protein precipitation, pH adjustment, and sonication can enhance the efficiency of the extraction process.
During elution, the choice of solvent and flow rate significantly affects recovery rates. A slower flow rate generally improves analyte retention, while multiple small elution volumes often yield better results than a single large volume. Additionally, optimizing the pH can help control ionization and improve separation in subsequent HPLC analysis.
Shanghai Haozhuang Instrument continues to innovate in the field of solid phase extraction, providing reliable and efficient tools that support accurate and consistent sample preparation across diverse industries. Whether for research, quality control, or clinical testing, their products are designed to meet the highest standards of performance and reliability.
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