Human zonulin ELISA Kit

**Human Zonulin ELISA Kit – For Quantitative In Vitro Determination of Human Zonulin in Serum, Plasma, Cerebrospinal Fluid, Tissue Homogenate, and Other Body Fluids** *For Laboratory Research Use Only. Not Intended for Diagnostic Procedures.* Before using this product, please read the entire package insert carefully. This ELISA kit is designed for research purposes only and should not be used in clinical diagnostics or therapeutic applications. The **Zonulin ELISA Kit** is based on a sandwich immunoassay principle. It allows for the accurate quantification of zonulin levels in various biological samples. The kit includes a set of calibration standards that are run alongside the samples to generate a standard curve. By comparing the optical density (OD) of the test samples to this curve, the concentration of zonulin can be precisely determined. --- ### **Sample Collection and Storage** - **Serum**: Collect blood in a serum separator tube. Allow clotting for 2 hours at room temperature or overnight at 4°C before centrifugation at 2000×g for 20 minutes. Remove the serum and assay immediately, or aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. - **Plasma**: Collect using heparin as an anticoagulant. Centrifuge within 30 minutes at 2000×g for 30 minutes at 2–8°C. Store at -20°C and avoid repeated freezing. - **Cell Culture Supernatants, Tissue Homogenates, and Other Biological Fluids**: Centrifuge to remove particulates and assay immediately or store at -20°C. Ensure no hemolysis or granules are present in the sample. --- ### **Materials Required but Not Supplied** 1. Incubator set at 37°C 2. Microplate reader capable of measuring absorbance at 450 nm 3. Precision pipettes, disposable tips, and absorbent paper 4. Distilled or deionized water --- ### **Reagents Provided (Storage: 2–8°C)** | Reagent | 96 Determinations | 48 Determinations | |--------|------------------|------------------| | MicroELISA Strip Plate | 12×8 strips | 12×4 strips | | Standard (6 vials) | 0.5 ml/vial | 0.5 ml/vial | | Sample Diluent | 6.0 ml | 3.0 ml | | HRP-Conjugate Reagent | 10.0 ml | 5.0 ml | | 20X Wash Solution | 25 ml | 15 ml | | Chromogen Solution A | 6.0 ml | 3.0 ml | | Chromogen Solution B | 6.0 ml | 3.0 ml | | Stop Solution | 6.0 ml | 3.0 ml | | Closure Plate Membrane | 2 | 2 | | User Manual | 1 | 1 | | Sealed Bags | 1 | 1 | *Standard concentrations: 2000, 1000, 500, 250, 125, 62.5 ng/mL. If sample values exceed the highest standard, dilute with Sample Diluent and repeat the assay.* --- ### **Precautions** 1. Do not mix reagents from different kit lots. All components are calibrated for optimal performance. 2. Bring all reagents and materials to room temperature (20–25°C) before use. Avoid using water baths for thawing. 3. Do not use reagents past their expiration date. 4. Use only deionized or distilled water for dilution. 5. Keep microtiter plates in sealed bags until ready for use. Store unused strips at 2–8°C with desiccant. 6. Use fresh pipette tips for each transfer to prevent contamination. 7. Treat all disposable items as potentially hazardous. Follow good laboratory practices. 8. Dispose of waste by adding hypochlorite to reach a final concentration of 1.0%. Let it sit for 30 minutes to inactivate viruses. 9. Substrate solutions are sensitive to contamination. Avoid exposure to light. 10. Chromogen B contains 20% acetone—keep away from heat and flame. 11. Allow all reagents to reach room temperature before use. --- ### **Reagent Preparation and Storage** - **Wash Solution (1X)**: Dilute 1 volume of 20X Wash Solution with 19 volumes of deionized or distilled water. Store at 2–8°C for up to one month. --- ### **Assay Procedure** 1. Prepare all reagents before starting. Run standards and samples in duplicate. 2. Add 50 µL of standard or sample to appropriate wells. Blank well: no addition. 3. Add 100 µL of HRP-conjugate reagent to all wells except blank. Cover with adhesive strip and incubate for 60 minutes at 37°C. 4. Wash the plate 4 times: - **Manual Washing**: Fill each well with 1X Wash Solution, aspirate, and repeat 4 times. Dry by blotting. - **Automated Washing**: Aspirate, wash 4 times with 1X buffer, adjust brush settings, and fill 350 µL per well. 5. Add 50 µL of Chromogen A and 50 µL of Chromogen B to each well. Mix gently and incubate for 15 minutes at 37°C, protecting from light. 6. Add 50 µL of Stop Solution to each well. Color changes from blue to yellow. If color is green or inconsistent, gently tap the plate. 7. Read OD at 450 nm using a microplate reader. --- ### **Data Interpretation** - Plot average OD (450 nm) of standards against their concentrations to create a standard curve. - Calculate mean OD for each sample and subtract blank value. - Locate the sample OD on the Y-axis, draw a horizontal line to the curve, then a vertical line to the X-axis to determine concentration. --- ### **Performance Characteristics** - **Intra-assay CV (%) and Inter-assay CV (%)**: <15% - **Assay Range**: 62.5 ng/mL – 2000 ng/mL - **Sensitivity**: <10 ng/mL - **Cross-reactivity**: No significant cross-reactivity observed - **Storage**: 2–8°C (frequent use); 6 months at -20°C --- **Note:** Always follow safety guidelines and proper disposal protocols. This kit is intended for research use only.

Office Chair

Office chairs can be used in companies, homes, and various commercial places. Our office chairs are comfortable to sit on, with many colors and varieties. The backrest is made of mesh cloth, and the cushion is made of sponge, breathable, dirt-resistant, suitable for office And games.Myopia in children is mostly caused by bad habits in the learning process, such as improper sitting posture and poor lighting. The learning environment created by a good learning table is still helpful for preventing myopia, so buy anti-myopia learning desks for children. There is still a certain effect, let children develop good study habits, which will accompany them for a lifetime.
After the child goes to school, they can use the study table. The study table on the market can adjust the height of the table according to the height of the child. The study table is an essential tool for children to do homework. For example, there is a great and handsome study table on the market. The function is intelligent lifting and lowering, and the desktop can be folded. , When a person sits in front of the table, they naturally enter the concave table top, and the two arms droop naturally. Because the two elbows are supported by the concave plate, the body naturally does not lean forward, which effectively solves the shortcomings of lying on the stomach to write and study. When shopping for a Study Desk, be sure to keep these must-have features in mind. The focus of the study table is to correct the sitting posture and prevent myopia, which is a foldable study table with a concave table top.

Pu Leather Office Chair,Office Chair,Nordic Office Chair,Stylish Office Chair

Igrow Technology Co.,LTD , https://www.igrowdesks.com